Recombinant Human Laminin-10 (α5β1γ1)

RECOMBINANT HUMAN LAMININ-10 (α5β1γ1): Production, Purification and Migration Promoting Activity on Vascular Endothelial Cells

Human recombinant laminin-binding protein: isolation, purification, and crystallization.

The mRNA of the precursor of laminin-binding protein (LBP) was isolated from a human embryo kidney cell line and cloned. The determined sequence of the LBP gene showed complete identity with the LBP genes isolated from human lung and large intestine cells. The human LBP was expressed by E. coli cells, and it was purified using Ni-NTA-Sepharose chromatography. The mobility of the homogeneous rec...

Integrin binding specificity of laminin-10/11

Interaction of epithelial and endothelial cells with basement membranes plays a critical role in induction and maintenance of their differentiation phenotypes. The biological activities of basement membranes can in large part be attributed to laminins, the major glycoproteins of basement membranes throughout the vertebrate body. Laminins are disulfide-linked heterotrimers of three distinct but ...

Recombinant human erythropoietin: 10 years of clinical experience.

The need for a renewable source of erythropoietin to treat the anaemia of chronic renal failure was first recognized in the 1960s, but cloning and expression of the human gene was not achieved until 1983. Clinical testing of recombinant human erythropoietin (r-HuEPO) began in 1985, leading to the first licence as a therapeutic agent in 1988. The first clinical trials showed that an intravenous ...

The production of recombinant human laminin-332 in a Leishmania tarentolae expression system.

Laminin (LM)-332 (alpha3beta3gamma2), a large heterotrimeric glycoprotein, is an essential component of epithelial basement membranes that promotes cell adhesion and migration. Here, we expressed human LM-332 using a novel protein expression system based on the trypanosomatid protozoan host Leishmania tarentolae. Plasmids containing cDNA encoding full-length beta3 and gamma2 subunits and trunca...